Lamiflo™ Enhances Blood Flow and Tissue Oxygenation
After Stroke and Traumatic Brain Injury.
Neuroprotection therapies for stroke and traumatic brain injury proven effective in animals all failed clinically. Lamiflo™ offers a new therapeutic approach by improving the physical dynamics of large artery and microvascular blood flow of tissue at risk.*
Lamiflo™ Improves Brain Blood Flow and Tissue Oxygenation After
Acute Ischemic Stroke (pMCAO) and Traumatic Brain Injury (TBI).
Fig.1) Studies in anesthetized rats, (n=10/group) subjected to permanent middle cerebral artery occlusion (pMCAO) or traumatic brain injury (TBI) studied by microscopy and a fluorescent dye. Variables recorded are percent arteriolar volume flow, perfused capillaries, NADH (lower values=better oxygenation) and blood brain barrier permeability (lower values=lower permeability). Lamiflo™ (2 ug/ml blood) or saline injected i.v. with dye flux via a temporal window insonating the cortical region viewed by 2PLSM at first use. two photon laser scanning microscopy ( 2PLSM). P< 0.05 for all Lamiflo™ and saline group comparisons.
Fig.2) DRP reduces neurodegeneration at 24 hours (A) Fluoro-Jade B stain and (B) improves neurologic outcome by Rotarod 1 week after TBI or pMCAO (N=5 rats per group). Microvascular flow and tissue oxygenation improved by Lamiflo™ (DRP) and tissue oxygenation reduced neurodegeneration by 37.6% (TBI) and 44.2% (pMCAO) compared to saline at 24 hrs by Fluoro-Jade staining, (P<0.05). One week postinsult Lamiflo™(DRP) treated rats performed Rotarod significantly better (P<0.05) (Fig. 2B) than controls.
Fig. 3A) 3-D vasculature reconstruction from Z-stack 2-Photon Laser Scanning Microscopy (2PLSM) images. Capillaries (3-8μm, green) differentiated from larger microvessel (8-20 μm diam, red). B. 2PLSM of rat cortex microvasculature with line scans settings for arteriole blood flow velocity profiles (red stripes). C. Arterial blood flow velocity profiles before [left] and after DRP injection [right, 140 μg/kg 1 hr postTBI] show increased flow velocity near vessel walls (140 μg/kg, 1 h post TBI, Δ,mm/s). D. Imaging volume from where every individual capillary flow was recorded. E. Line scans from the capillary before [up] and after [low] showed increased velocity with DRP. F. Frequency histogram show flow velocity increase after DRP injection. G. Dose effect on capillary flow in male and female rats (for black n=10, for red =3,*P<0.05 Mean Å} SEM).
* Bragin DE, Kameneva MV, Bragina OA, Thomson S, Statom GL, Lara DA, Yang Y, Nemoto EM. Rheological effects of drag-reducing polymers improve cerebral blood flow and oxygenation after traumatic brain injury in rats. J Cereb Blood Flow Metab. 2017 Mar;37(3):762-775.
Bragin DE, Peng Z, Bragina OA, Statom GL, Kameneva MV, Nemoto EM. Improvement of Impaired Cerebral Microcirculation Using Rheological Modulation by Drag-Reducing Polymers. Adv Exp Med Biol 2016;923: 239-244. doi: 10.1007/978-3-319-38810-6_32.PMID: 27526149