Neuroprotection for Stroke and Traumatic Brain Injury

Fig.1) Studies in anesthetized rats, (n=10/group) subjected to permanent middle cerebral artery occlusion (pMCAO) or traumatic brain injury (TBI) studied by microscopy and a fluorescent dye. Variables recorded are percent arteriolar volume flow, perfused capillaries, NADH (lower values=better oxygenation) and blood brain barrier permeability (lower values=lower permeability). Lamiflo™ (2 ug/ml blood) or saline injected i.v. with dye flux via a temporal window insonating the cortical region viewed by 2PLSM at first use. two photon laser scanning microscopy ( 2PLSM). P< 0.05 for all Lamiflo™ and saline group comparisons.

Fig. 3A) 3-D vasculature reconstruction from Z-stack 2-Photon Laser Scanning Microscopy (2PLSM) images. Capillaries (3-8μm, green) differentiated from larger microvessel (8-20 μm diam, red). B. 2PLSM of rat cortex microvasculature with line scans settings for arteriole blood flow velocity profiles (red stripes). C. Arterial blood flow velocity profiles before [left] and after DRP injection [right, 140 μg/kg 1 hr postTBI] show increased flow velocity near vessel walls (140 μg/kg, 1 h post TBI, Δ,mm/s). D. Imaging volume from where every individual capillary flow was recorded. E. Line scans from the capillary before [up] and after [low] showed increased velocity with DRP. F. Frequency histogram show flow velocity increase after DRP injection. G. Dose effect on capillary flow in male and female rats (for black n=10, for red =3,*P<0.05 Mean Å} SEM).